"A psychiatrist tells two stories: one of a trans woman, the other of a pseudohermaphrodite."
Bela Lugosi
as Scientist
Edward D. Wood Jr.
as Glen / Glenda (as Daniel Davis)
Lyle Talbot
as Inspector Warren
Timothy Farrell
as Dr. Alton / Narrator
Dolores Fuller
as Barbara
'Tommy' Haynes
as Alan / Anne
Charlie Crafts
as Johnny (as Charles Crafts)
Conrad Brooks
as Banker / Reporter / Pickup Artist / Bearded Drag (as Connie Brooks)
Henry Bederski
as Man with Hat and Receding Hairline (uncredited)
Carol Daugherty
as Woman in Nightmare (uncredited)
'Captain' DeZita
as The Devil / Glen's Father (uncredited)
Helen Miles
as (uncredited)
Shirley Speril
as Miss Stevens (uncredited)
Amzie Strickland
as Minor Role (uncredited)
Harry Thomas
as Man in Nightmare (uncredited)
William C. Thompson
as Judge (uncredited)
'Mr. Walter' Hadjwiecyz
as Patrick / Patricia (uncredited)
George Weiss
as Man at Transvestite's Suicide (uncredited)
Evelyn Wood
as Sheila, Glen's Sister (uncredited)
Director
Director of Photography
Producer
Story
Screenplay
Sanford H. Dickinson
Music Supervisor
Ben Winkler
Sound Recordist
Bud Schelling
Editor
Scott McCloud
Second Unit
Makeup Artist
Jack Miles
Settings
Bert Shipman
Camera Operator
Larry Smith
Still Photographer
Nathan Bailey
Medical Consultant